This research project has the aim to study the interaction between the tulip bulb and the fungus by visualizing the interaction by using microscopy. Therefore the techniques developed for the FusariumScreen of Plant Research International will be used.
This Fusarium Screen was developed to study the interaction of fusarium in grains. This method will be adjusted for Fusarium in tulip. Therefore the following steps are needed:
- Transformation of virulent and less-virulent strains of F. oxysporum f.sp. tulipae will be transformed with GFP that will be constitutive expressed.
- Adjustment of the Fusarium Screen to study the interaction between Fusarium and tulip
After establishing a good Fusarium Screen for fusarium in tulip factors influencing the interaction between Fusarium and tulip will be studied. These factors include harvesting moments, conditions during processing of the bulbs, different conditions during storage of the bulbs, latent infections and ethylene.
2009Set of characterized GFP/RFP transformants of several virulent and less virulent strains of F. oxysporum f.sp. tulipae
Fusarium Screen adjusted for tulip
- First comparison on the interaction between virulent or less virulent strains of F. oxysporum on susceptible or less susceptible tulip cultivars.
- Conclusion on factors influencing the development of Fusarium in tuli.
Controlling (a part of) the factors influencing the development of Fusarium in tulip.
Fusarium oxysporum f. sp. tulipae causes a devastating dry rot of tulip bulbs during storage. Losses are estimated at approximately 10 million € every year. The practical solutions to prevent this dry rot are mainly focused on the prevention of favorable conditions for infection of the fusarium spores. In spite of taking different measures to prevent these conditions fusarium rot of tulips still lead to great losses the past years. Therefore the tulip growers, bulb export companies and research believe the interaction between tulip bulbs and the fungus F. oxysporum f.sp. tulipae should be characterized thoroughly. This way effective and practical solutions supplementary to the measures that are already applied can be developed. This will lead to a chain management advice for controlling Fusarium dry rot in tulips.