Animesh Acharjee: Systems biology and statistical data integration of ~omics data sets

Promovendus Animesh Acharjee
Promotor prof.dr. RGF (Richard) Visser
Copromotor CA (Chris) Maliepaard
Organisatie Wageningen University, Wageningen Plant Breeding, Laboratory of Plant Breeding

wo 12 juni 2013 13:30 tot 15:00

Locatie Auditorium, building number 362
Generaal Foulkesweg 1
6703 BG Wageningen

In this thesis quality traits of potato were related to different highly multivariate ~omics datasets containing information on proteins, primary and secondary metabolites and gene expression. The objectives were to explore and compare different statistical techniques that are able to quantify these relationships, and to identify components responsible for prediction of quality. We propose a strategy to integrate two or more of such datasets and to select subsets of predictive components. We used potato flesh colour as an example trait and identified metabolites and expressed genes that are associated with flesh colour. We identified two putative novel non-volatile glycosides of carotenoid-derived metabolites and a novel putative connection with the flavonoid pathway. From a gas chromatography data set we identified genetic factors underlying variation in primary metabolism and found the amino acid beta-alanine associated with starch content. Finally we performed an integrated analysis with gene expression, metabolites and proteomics data and present an approach to select a limited set of predictive genes, metabolites and proteins.

An untargeted ~omics data set is like a book in an unknown language: the number of words can be impressive but the story needs an interpreter