The present-day cultivars of button mushroom are too sensitive to mechanical damage. Consequently, mushrooms for the fresh market are still handpicked, which is a main cost factor for the Dutch mushroom industry.
The main objective of this thesis is to use the phenotypic variation and segregation to study the genetic determinants explaining the differences in bruising sensitivity and generate breeding stocks that can be used to construct commercial varieties that are suitable for mechanical harvest. In addition, the genetic basis of other quality-related traits was investigated. For this, segregating populations were generated and used to study in more detail the life cycle of the bisporic variety. The availability of the whole genome sequence allows a more detailed study of recombination frequencies generating knowledge invaluable for further mushroom breeding.