Matrix modulation of the toxicity of alkenylbenzenes studied by an integrated approach using in vitro, in vivo and physiologically based biokinetic (PBBK) models

The PhD research aimed at investigating the protective effect (matrix effect) exerted by some herbal ingredients against the hepatocarcinogenicity of alkenylbenzenes which are important flavour molecules present in herbs and spices.

Promovendus WAAM (Wasma) Al-Husainy MSc
Promotor IMCM (Ivonne) Rietjens
Copromotor Ans Punt
Copromotor prof. dr PJ van Bladeren
Organisatie Wageningen University, Sub-department of Toxicology

di 10 december 2013 11:00 tot 12:30

Locatie Auditorium, building number 362
Generaal Foulkesweg 1
6703 BG Wageningen

The inhibition was quantified in vitro and thereafter data were integrated into the so-called physiologically based biokinetic (PPBK) models to predict the reduction in adverse effects of alkenylbenzenes in the liver of human and rat when the protective ingredients would be dosed in rodent bioassays together with the alkenylbenzenes. Inhibition of adverse effects of alkenylbenzenes shown in the in vitro experiments and predicted by our PPBK models was also validated in vivo using male rats.

The results from this PhD research indicate that the likelihood of adverse effects may be lower in rodent bioassays when alkenylbenzenes would be dosed in a matrix containing active ingredients from herbs and spices compared to experiments using pure alkenylbenzenes as single compounds.