In spring 2013, CAT-AgroFood has invested in a PamStation®12 of PamGene, that is installed at the Toxicology sub-department of Wageningen UR. The instrument can for instance be used for pathway elucidation, toxicology insight, biomarker discovery and a broader spectrum of possibilities and is accessible for researchers of Wageningen UR, as well as for researchers from companies, knowledge institutes and universities.
Arjé Brilleman (Director Sales Europe, PamGene) and prof.dr. John Groten (managing director PamGene and part-time professor Combination Toxicology at Wageningen UR) will present the versatility of the platform and they will explain how PamGene’s application could be used and support you in your research.
Kinase activity platform
Contrary to other methods, kinase activity is measured and not just kinase presence. One and the same method can be used for recombinant kinases, cell lines, yeast, zebrafish xenografts, and clinical samples. As input 1000 to 10000 cells per assay (0.5 – 5 ug of protein per assay) is sufficient, to generate hypotheses about kinases and pathways active under certain conditions.
Nuclear receptor platform
By investigating the broad spectrum of interactions of these receptors with multiple cofactors present on the PamChip, leads to further elucidation of normal and aberrant gene transcription. The functional readout is especially relevant in investigations of ligand and drug profiling, as both potency, efficacy and selectivity are monitored concomitantly.
Beyond using purified proteins, it is now possible to test for receptor functionality in crude cell lysates, and even tumor tissue extracts from breast cancer patients.
Brief background about PamGene:
PamGene, an innovative biotechnology company in The Netherlands, has developed a new technology which enables researchers to measure tyrosine and serine/threonine kinases activity and/or monitor the functional properties (activity) of nuclear receptors (NR) in recruiting cofactors. The technology is used in a variety of applications, from basic research to applied pharmaceutical sciences.