Publication: A rapid, sensitive and cost-efficient assay to estimate viability of potato cyst nematodes

Gepubliceerd op
5 oktober 2011

DNA is a relatively stable polymer, and that one of the main reasons why DNA technology is more and more used for forensic purposes. It is also employed to detect plant pathogens with a quarantine status. Some pathogen species have specific life stages for the survival of adverse conditions ( DNA is well-protected), and particularly for these ones it could happen that a positive DNA signal is actually derived from a dead individual.


Potato cyst nematodes, Globodera rostochiensis  and G. pallida, are quarantine organisms. Van den Elsen and co-workers developed a method that allows for a rapid and cheap determination of vitality of cysts that can easily be combined with a DNA-based species determination.