Si Wang: Toxicogenomics-based in vitro alternatives for estrogenicity testing

Testing chemicals for their endocrine-disrupting potential, including interference with estrogen receptor signaling, is an important aspect to assess the safety of currently used and newly developed chemicals.

Promovendus Si Wang
Promotor IMCM (Ivonne) Rietjens
Copromotor TFH (Toine) Bovee JMMJG (Jac) Aarts
Organisatie Wageningen University, Sub-department of Toxicology

do 20 juni 2013 11:00 tot 12:30

Locatie Auditorium, building number 362
Generaal Foulkesweg 1
6703 BG Wageningen

The standard test for disruption of normal estrogen function is the in vivo uterotrophic assay in immature or ovariectomised rodents with uterus weight as a crucial read-out parameter. Due to the high costs, ethical objections and labour intensiveness of the in vivo uterotrophic assay, the development of an in vitro (in glass) test battery for in vivo estrogenicity has high priority. The present thesis provides proof-of-principle that combining in vitro assays measuring different steps in the estrogen receptor signaling pathway enables accurate prediction of the estrogenic effects in vivo.

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