Valorization of jatropha fruit biomass for energy applications

Promovendus mr. A Marasabessy
Promotor prof.dr. JPM (Johan) Sanders
Copromotor dr RA Weusthuis
dr MR Moeis
Organisatie Wageningen University

do 2 april 2015 11:00 tot 12:30

Locatie Auditorium, building number 362
Generaal Foulkesweg 1
6703 BG Wageningen


Microbial extraction of oil from Jatropha kernels using whole cells of Bacillus pumilus yields 73% oil. The oil is liberated  via degradation of hemicelluloses in which the majority kernel proteins were preserved. In investigating the effect of dilute sulfuric acid pretreatment on the enzymatic digestibility of the lignocellulosic components of Jatropha fruit biomass, we found that the seed shell and the seed cake were more recalcitrant to dilute sulfuric acid pretreatments than the fruit hull. The fruit hull pretreated with dilute sulfuric acid at optimum conditions (10% solid loading, 0.9% sulfuric acid, 30 min, 178 oC) followed by neutralization and a 24-h enzymatic hydrolysis with cellulases (GC220) gave the hydrolyzate with high sugar content. The hydrolyzate can be used as a substrate to produce ethanol by Saccharomyces cerevisiae in SSF process.