In the battle against malaria, Wageningen Food & Biobased Research worked together with partners from Africa and Europe on a research project to assess the specific markers and develop innovative, rapid and simple diagnostics. This project is funded by the European Commission (contract Health-F3-2008-201889).
In collaboration with the Royal Tropical Institute the group BioSensing & Diagnostics developed a PCR - lateral flow test, the Nucleic Acid Lateral Flow ImmunoAssay (NALFIA), for the detection of specific malaria amplicons. The 4-lines NALFIA detected Pan-Plasmodium (all Plasmodium species), P.vivax, P.falciparum and a human gene specific amplicons (test control). The test was evaluated in several human trials in Africa and Asia (Mens et al., 2012a,b).
A NALFIA for the detection of single nucleotide polymorphisms (SNPs) in relation to resistance to drug combination therapy was developed as well (Moers et al., 2015)