I conducted, my master thesis in Wageningen University, Netherlands in collaboration with Gent University, Belgium. The main focus was in plant-nematode interactions with the aim of gaining a comprehensive view of plant defence signaling in Arabidopsis thaliana following infection with root-knot nematodes and how nematodes manipulate plant hormones to enhance infection.
A series of mutant and transgenic lines altered in specific hormone pathways were challenged with and evaluated for susceptibility and Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) used for differential gene expression analysis of selected defence related genes. In the Laboratory of Nematology, WUR where I worked for three months under the supervision of Assistant prof. Aska Goverse and Casper van Schaik, I was able to show the importance of auxin hormone for root-knot nematodes to cause infection and that plants use Jasmonic acid (JA) and salicylic acid (SA)to protect themselves against nematode attack. My experience in Wageningen was amazing to say the least, the lab is well furnished with many modern equipments and the level of organization is indescribable. The working atmosphere is truly nice with very helpful people and I got to learn a lot of techniques through their help. For sure I carry with me many memorable moments from Wageningen and sure recommend the nematology group as the best place to be.