Studentenverhaal

Testimonial David Braspenning

As Msc student Plant Sciences I did a minor thesis at the Nematology department. My prior bachelor Plant Sciences consisted of a lot of master courses. Therefore I did almost all my obligatory courses for my Master already and had some space left in my study program for a second thesis. My supervisor Erin Bakker introduced me in the field of plant nematode interactions which I found very interesting.

I was looking for functional and non-functional orthologs of Gpa2 to determine which amino-acids are required for recognition of Rbp-1 by the Gpa2 R-protein
David Braspenning

June 2012

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My thesis was about the interaction of the Solanum resistance gene Gpa2 and the effector Rbp-1 from potato cyst nematode Globedera pallida. I was looking for functional and non-functional orthologs of Gpa2 to determine which amino-acids are required for recognition of Rbp-1 by the Gpa2 R-protein. Therefore I performed lots of PCRs in the lab and lots of Agrobacterium transient assays in the glasshouse.

I experienced a very nice working atmosphere at Nematology due to the people working here. The people in the laboratory were often so kind to help explaining equipment and techniques. I enjoyed my time working in the “spit lab”  were it was nice working, because of the social and helpful people there. Besides that there was often cake for some celebrations and the Christmas activity I joined was very nice. Thanks a lot!