For Master Thesis my Subject was about; Identification of candidate genes for the potato cyst- nematode resistance locus Grp1. Based on previous research, Sequence information from the diploid potato clone RH showed the presence of six different Resistance gene homologue clusters in the Gp21-Gp179 interval on chromosome V.
Therefore this study majorly focused on answering the question to which of these Resistance gene homologue (RGH) clusters belongs the Grp1 gene, by studying two of the six clusters and finding out the one which is most closely linked to the Grp1 locus. This was under the Supervision of Dr. Erin Bakker. During period of three (3) months at Laboratory of Nematology WUR, I developed Cluster specific markers for RGH cluster Ve and Vf, with Cleaved amplified polymorphic sequences (CAPs) and Locked nucleotide acid (LNA) for the mapping population and mapped markers in high- resolution map of Grp1 locus.
It is from this Laboratory that, I gained good practical knowledge, skills and competence in molecular work especially aspects of Molecular marker assisted selection which has been my major interest of learning. I can therefore confirm a quote in my culture which says “a boy who only eat his mothers’ food, think his mother is the best cook”. Laboratory of Nematology is well organized, making it a good working environment. Lastly I would like to appreciate the level of cooperation staff members give to their students.