At the end of my bachelor I had to do my bachelor thesis. Due to the good experience I had with Arjen’s supervision during the course Host-Parasite interactions and the subject, I choose to do my thesis at the Laboratory of Nematology.
My direct supervisor, Lotte, offered me a lot of papers about the subject and I started reading. After taking up all new information I talked with Lotte to shape my research project. My focus was on the production T2 RNAses in N. benthamiana by using A. tumefaciens and to purify these proteins from the plant material. The genes expressing these proteins were codon optimized for N. benthamiana and a signal peptide was added to ensure secretion of the products into the apoplast. This secretion makes the purification easier, thereby it leads to a higher yield. The T2 RNAse activity of the purified enzymes was assessed. After this assessment my time at the Laboratory of Nematology was over. A new student will continue with the research where I left of. The idea that my hard work and my thesis were not just for the ECTS, but also to give a small contribution to science was one of the things I still like when I think about the time at NEM. Besides, all the people I’ve worked with during my thesis were friendly and I really enjoyed my time with them.