PhD Super-resolution microscopy

Gepubliceerd op
23 juli 2018
Locatie Wageningen
Expertisegebied Natural Sciences
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We are looking for

We are looking for a PhD candidate for the work package “Localisation of oxidation: Interfaces and transport”, which is part of the NWO project titled LICENSE (Lipid oxIdation Control Enabled by Natural StratEgies). LICENSE is a collaboration between Wageningen University, University of Amsterdam, Eindhoven University of Technology and various industrial partners offering in total 7 PhD positions. The goal of LICENSE is to understand and control the mechanisms behind lipid oxidation in food emulsions and identify novel anti-oxidant strategies.

The aim of your PhD project is to develop fluorescence-based imaging techniques that allow the quantitative assessment of the multi-scale nature of lipid oxidation. We are specifically interested in employing super-resolution microscopy, which has been primarily applied in the biomedical field, to resolve structural features at the 10 nm length scale in food emulsions. These emulsions, however, are barely transparent and the obtainable spatial and temporal resolution is therefore limited due to aberrations, scattering and absorption. To overcome these issues, you will have the chance to develop and build a new advanced microscope (#miCube) that features adaptive optics, which can improve the performance of optical systems, and lasers operating in the near infrared region to increase the penetration depth. Further, you will investigate different labelling strategies to monitor lipid oxidation in the samples provided by our industrial partners. For accessing larger length scales, confocal scanning light microscopy (µm-mm scale) or MRI/ESR imaging facilities are available. Within your work package, you will work closely with a PhD student in Eindhoven who focusses on developing correlative electron microscopy approaches. Together you will deliver a multiscale view on lipid oxidation in food emulsions to the LICENSE project.

We ask

We seek a candidate with a MSc degree in (Bio-)Physics, (Mechanical or Optical) Engineering, (Physical or Analytical) Chemistry, Nanotechnology or similar to join our team. The ideal candidate has experience in quantitative microscopy or has set up scientific instrumentation, has experience in a wet-lab (sample preparation), is fluent in English, and enjoys working on original and fundamental research in an interdisciplinary team. Experience with super-resolution microscopy, single-molecule spectroscopy or software development (programming) is preferred but not required. An interview and a scientific presentation are part of the selection process. 

We offer

We offer you a temporary position for a period of 1.5 years with an extension of 2.5 years after successful evaluation. Gross salary is € 2266,- per month in the first year rising up to € 2897,- per month in the fourth year. We not only offer a competitive salary but also good (study) leave and a pension of the ABP Pension Fund.

More information

For more information about this position and the group, please contact Dr. Johannes Hohlbein via email ( or Twitter (@HohlbeinLab) or visit

You can apply online at until 20th of August 2018.

Your application should contain, (a) a letter of motivation including a reference to the advertised position, (b) a short CV including final grades of followed courses, and (c) contact details of former supervisors that can provide a recommendation letter.

We are

The Laboratory of Biophysics (BIP) engages in research of the physical aspects of life processes and teaching of the fundamentals of physics and biophysics and their application in the life sciences. The research of BIP relies on both optical and magnetic resonance techniques. BIP is a dynamic international team comprising around 11 staff members, 9 PhD students and post-docs. More information on our team can be found at BIP is embedded in the BioMolecular Science cluster and participates in the MAGNEtic resonance research FacilitY (MAGNIFY) and the MicroSpectrocopy research faCility (MSC).

The Agrotechnology & Food Sciences Group is part of Wageningen University & Research, an organisation that brings together fundamental and applied scientific research. We work on creating ‘healthy food in a biobased society’ with private sector companies, research institutes and government agencies in the Netherlands and abroad. Our innovations play a crucial role in solving the major issues which affect society today. Entrepreneurship and professionalism are key in all that we do. In short, we are a major international employer offering considerable potential for your career.

Wageningen University & Research
Delivering a substantial contribution to the quality of life. That's our focus – each and every day. Within our domain of good and safe food & food production, food security and a healthy living environment, we search for answers to issues affecting society – such as sustainable food production, climate change and alternative energy. Of course, we don’t do this alone. Every day, 5000 people work on ‘the quality of life’, turning ideas into reality, on a global scale.

Could you be one of these people? We give you the space you need.

For further information about working at Wageningen University & Research, take a look at:

Acquisition regarding this vacancy is not appreciated.