(Sub)Cellular Imaging
Range of fluorescence microscopes is available for spectroscopy on micrometre scale. Proces-
ses in compartments of cells can be imaged.
Our equipment
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Confocal Laser Scanning Microscopy (CLSM)
Confocal laser scanning microscopy (CLSM) allows 3D localization of labelled target molecules in cells and food samples. -
FRET-FLIM (Förster resonance energy transfer by fluorescence lifetime imaging)
To image FRET between proteins in root cells, the Leica SP5 CLSM is equipped with fluorescence lifetime imaging (FLIM).
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Fluorescence correlation spectroscopy (FCS)
FCS measures diffusion, dynamics and spatial distribution of single fluorescent molecules in solution and in living cells. -
Fluorescence Recovery After Photobleaching (FRAP)
FRAP is used to quantify diffusion of fluorescently labeled probes in cells. -
Total internal reflection fluorescence microscopy (TIRF)
Dynamics and interactions of labelled molecules in the plasma membrane of cells (that adhere to the glass surface) can be imaged with high sensitivity...