Vector map
The pMF1 vector (Figure 3) is equipped with the npt II gene for kanamycin selection of transgenic shoots.
Two additional pMF-versions, pMF2 and pMF3 are available in which the selection gene hpt (hygromycin resistance) and pat (phosphinothricin resistance), respectively, have been introduced into a unique Spe I-restriction site of pMF1. It is also possible to introduce reporter genes like gus or gfp at the Spe I-site for monitoring initial transformation efficiencies. All selection genes and reporter genes will ultimately be removed.
RB, LB | Right and Left Border of T-DNA, respectively |
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Rs | Recombination Site |
rec-LBD | Fusion of Recombinase R-gene and Ligand Binding Domain |
cod A-npt II | Fusion of cod A gene for negative selection on fluoro cytosine and npt II gene for positive selection on kanamycin |
SpeI | Restriction site in which hpt II or pat genes have been inserted in pMF2 and pMF3, respectively |
MCS | Multiple Cloning Site |