Caesarean Derived, Colostrum Deprived (CDCD), specific pathogen free (SPF), small intestinal segment perfusion test (SISP) CRO Bioveterinary

Surgical techniques

Wageningen Bioveterinary Research (WBVR) has high-tech operation rooms where complex surgical procedures can be performed by our excellent biotechnical experts. Livestock can be subjected to complex procedures like the production of caesarean-derived, colostrum-deprived animals (CDCD) and small intestinal segment perfusion in pigs.

Caesarean-derived, colostrum-deprived animals

We make use of CDCD animals for different types of studies. Since CDCD animals are not born via the vaginal route, they cannot be contaminated with microorganisms via the vaginal transmission route. This guarantees that the animals are free of specified pathogens. In addition, CDCD piglets are obtained from specific pathogen free (SPF) mother animals. By depriving animals of colostrum, transmission of maternal antibodies is prevented. Pathogens are kept out by scrupulously monitoring, disinfecting and filtering everything that comes into contact with the CDCD animals. This results in animals with a "clean" microbiological status that are immunologically naive to all pathogens. For vaccination trials this can be a big advantage. We can produce CDCD calves as well as piglets.

Small intestinal segment perfusion

The small intestinal segment perfusion test (SISP) is a porcine model for intestine-related questions. A weaned piglet is sedated to allow opening of the abdominal cavity and the small intestine is divided into in 10 segments. Each segment is about 20 cm long and cannulated at both ends with a cranial inflow and a caudal outflow. These canula are used for perfusion and to monitor the secretion of fluids by the intestine. Each segment can be used to test an intervention (infection, feed components, medicine) and determine the effects of the intervention on secretion of fluids (diarrhoea) and on host responses by studying gene expression.