The role of extra-column volume in column efficiency and upscaling

Schultze-Jena, A.S.C.; Boon, M.A.; Bussmann, P.J.Th.; Janssen, A.E.M.; Padt, A. van der


•Column efficiency is higher when extra-column volume is not taken into account
•Column dimensions could be up to 20% underestimated
•The term efficiency loss is misleading in preparative chromatography