The efforts of Bolivia’s water sector to adapt to climate change include the mainstreaming of adaptation in water policy instruments and broad capacity building processes supported by climate funds and international cooperation. These sector-wide adaptation experiences in the country present important learning challenges across different governance levels. This paper analyzes multi-level learning in the governance of adaptation taking place in the water sector in Bolivia, by focusing on changes in the cognitive, normative and relational domains of learning. The analysis is guided by three questions: (i) Which institutional arrangements enable multi-level learning in the governance of adaptation in Bolivia’s water sector? (ii) What are the cognitive, normative and relational dimensions of learning in these arrangements? (iii) What are the implications of multi-level learning for shaping desired outcomes in the governance of adaptation? The case contributes to understanding multi-level learning processes in the governance of adaptation, including the role of national and international climate change policy instruments in these. In addition, the study provides methodological insights for assessing multi-level learning.