In Lelystad we have a specialized fish and shellfish diseases laboratory. This is the one complete diseases laboratory in the Netherlands for cultured fish for human consumption, ornamental and wild fish and shellfish.
Fish production via aquaculture is increasing strongly worldwide. One of the threats to intensive aquaculture is the occurrence of contagious fish, crustaceans and shellfish diseases, which can cause serious illness and death and thereby economic losses.
Role Wageningen Bioveterinary Research
At the specialized fish and shellfish diseases laboratory of Wageningen Bioveterinary Research, accredited and fast diagnostics are performed to identify new outbreaks at an early stage. This is the one complete diseases laboratory in the Netherlands for cultured fish for human consumption, ornamental and wild fish and shellfish. We thus make an important contribution to healthy fish in healthy aquaculture and to safe and healthy fish products for human consumption.
Our core tasks
Wageningen Bioveterinary Research has broad experience and expertise on various fish and shellfish diseases. We are consulted frequently for our diagnostic service,expertise on diseases, and as partner for collaboration in research and network projects by both national and international parties.
The fish diseases laboratory was set up in 1985, and the task on shellfish diseases was added in 2000. The laboratory has the following core tasks:
- Serving as a knowledge and advisory centre for the Dutch Ministry of Agriculture, Nature and Food Quality (LNV);
Diagnosing diseases for the aquatic industry, and the ornamental and wild fish branches:
- In case of fish disease at fish farms
- At suspicion of notifiable fish and shellfish diseases, in cooperation with the Food and Consumer Product Safety Authority (Voedsel en Waren Autoriteit)
- For certification of live fish for export, in cooperation with the Food and Consumer Product Safety Authority (NVWA).
- Provide expertise by consultancy for the government and the aquaculture branch regarding fish and shellfish diseases
- Research on fish and shellfish diseases that occur in the Netherlands.
National Reference Lab
The laboratory is the Dutch National Reference Lab (NRL) for fish and shellfish diseases for the EU. It was the first fish diseases laboratory in Europe accredited for quality standards ISO 9001 and 17025 (EN 45001). The laboratory has an impressive national and international network, in which knowledge and materials are exchanged.
The inoculated agar plates are incubated at room temperature, as fish are cold-blooded animals and their pathogenic organisms have adapted to that temperature. In case a pure culture of bacteria results, an antibiogram is made, so that the fish owner can choose an appropriate antibiotic to treat the disease. In parallel to this process, the bacteria are characterized by classic and/or molecular techniques.
For virus isolation, a suspension of the fish organs is put on fish cell cultures to cultivate any virus that may be present. The morphology of the virus can be examined with an electron microscope. The virus is generally characterized with specific antibodies that neutralize the particular virus. Moreover, for various viruses PCR-tests (sensitive rapid diagnostics) are available. After the name of the virus is known, specific recommendations are made, for example regarding changes in the water temperature in the fish culture system, to inhibit clinical disease.
For histopathology, the fixed tissue is sliced and stained in such a way that it can be examined with a microscope. This makes it possible to see alterations at cell level that indicate a viral, bacteriological or other disease. This method also enables detection of very small parasites in the tissue or in cells.
The alloherpesviral counterparts of interleukin 10 in European eel and common carp.
Fish and Shellfish Immunology 31 (2011)6. - ISSN 1050-4648 - p. 1211 - 1217.
Identification and localization of the structural proteins of anguillid herpesvirus 1
Veterinary Research 42 (2011). - ISSN 0928-4249 - 15 p.
Antibody coated goldnanoparticles immunoassay for direct detection of Aeromonas salmonicida in fish tissues.
Journal of Fish Diseases 34 (2011)11. - ISSN 0140-7775 - p. 845 - 852.
Development and validation of a two-step real-time RT-PCR for the detection of eel virus European X in European eel, Anguilla anguilla.
Journal of Virological Methods 171 (2011)2. - ISSN 0166-0934 - p. 352 - 359.
Zoonose Vibrio vulnificus: meldingsplicht raadzaam
Nederlands Tijdschrift voor Geneeskunde 2010 (2010)154. - ISSN 0028-2162 - p. A2320 - A2320.
The health status of European silver eels, Anguilla anguilla, in the Dutch River Rhine Watershed and lake IJsselmeer
Aquaculture 309 (2010)1-4. - ISSN 0044-8486 - p. 15 - 24.
Epidemiology of Bonamia ostrea infecting European flat oysters Ostrea edulis from Lake Grevelingen, The Netherlands
Marine Ecology Progress Series 409 (2010). - ISSN 0171-8630 - p. 131 - 142.
Complete genome sequence and taxonomic position of anguillid herpesvirus 1
Journal of General Virology 91 (2010)4. - ISSN 0022-1317 - p. 880 - 887.
Inter-relationships of haplosporidians deduced from ultrastructural studies
Diseases of Aquatic Organisms 83 (2009)3. - ISSN 0177-5103 - p. 247 - 256.
Differentiation between Cyprinid herpesvirus type-3 lineages using duplex PCR
Journal of Virological Methods 158 (2009)1-2. - ISSN 0166-0934 - p. 51 - 57.