BSc and MSc Thesis Subjects of the Bioinformatics Group
On this page you can find an overview of the BSc and MSc thesis topics that are offered by our group. The procedure to find the right thesis project for you is described below.
MSc thesis: In the Bioinformatics group, we offer a wide range of MSc thesis projects, from applied bioinformatics to computational method development. Here is a list of available MSc thesis projects. Besides the fact that these topics can be pursued for a MSc thesis, they can also be pursued as part of a Research Practice.
BSc thesis: As a BSc student you will work as an apprentice alongside one of the PhD students or postdocs in the group. You will work on your own research project, closely guided by your supervisor. You will be expected to work with several tools and/or databases, be creative and potentially overcome technical challenges. Below you will find short descriptions of the research projects of our PhDs and Postdocs. In addition you can take a look at the list of MSc thesis projects above.
Procedure for WUR students:
- Request an intake meeting with one of our thesis coordinators by filling out the MSc intake form or BSc intake form and sending it to thesis@bioinformatics.nl
- Contact project supervisors to discuss specific projects that fit your background and interest
- Upon a match, take care of the required thesis administration together with your supervisor(s) and enroll in the thesis BrightSpace site to find more information on a thesis in the Bioinformatics group
Procedure for non-WUR students or students in other non-standard situations: email our thesis coordinators at thesis@bioinformatics.nl to discuss your interests and the possibilities.
BSc thesis topics
Integrative omics for the discovery of biosynthetic pathways in plants
I am a postdoctoral researcher at Wageningen University and Research. My research interests include exploring omics (Genomics, Transcriptomics and Metabolomics) data to understand patterns and mechanisms of plant biotic interactions and co-evolution. In my current project, I explore and implement innovative omics integration strategies to map genes and their expression patterns to metabolites that play key roles in plant-endophyte interactions. My previous projects were mainly driven by key questions in resistance evolution of insect-pests to natural and synthetic insecticides. I used genome-wide investigations and computational approaches (mainly genome informatics, phylogenomic and population genomics) to answer such questions, ensuring these are relevant to a broad scientific community.
Molecular function prediction of natural products
Organisms like microbes, plants, and sponges produce a variety of secondary metabolites which they use to interact with the world around them. These secondary metabolites, also called natural products, often have potent bioactivities – which makes them ideal as (resource for) pharmaceuticals. The biosynthetic machinery coded in the genomes of these organisms responsible for producing these molecules is often highly conserved in separate clumps of genes, i.e. biosynthetic gene clusters. My research focusses on inferring molecular structures from such biosynthetic gene clusters in order to link genomic to metabolomic data. Additionally, we can use these inferred chemical spaces to predict the bioactivity of these compounds (e.g., by using graph neural networks). These efforts will advance knowledge on how to target and engineer interesting biosynthetic machinery more effectively.
From gene to networks: bridging the gap between two temperature-regulated plant reproductive traits
Both ambient temperature-controlled flowering time and seed dormancy setting are temperature-regulated traits, which have been recently proposed to be interconnected. Plant reproductive success is the result of the evolution of complex gene regulatory networks. A challenge is to interconnect the variety of publicly available information, such as transcriptomic and genome-wide chromatin immunoprecipitation data sets. We hypothesize that the plant reuses the same regulatory modules throughout development. For instance, the same transcription factor, can positively or negatively regulate a set of genes depending on its differential interaction with other proteins.
In this project, you will perform a network analysis starting from a set of 306 genes known to be relevant for flowering, and from publicly accessible gene expression data of Arabidopsis fruit tissue. Initially, a search for available high quality data-sets will be performed, followed by network analyses. Prior knowledge will be used to evaluate predictions and to improve the accuracy in estimating the gene network structure. The ultimate goal is to build a highly connected network and to identify potential hubs in it. Potentially, once the network is fully built and validated, it can be further used for predicting relevant genes and their functions.
Genome-guided discovery and structure prediction of novel bio-surfactants
Surfactants are integral compounds found in cosmetics, industrial cleaners, and food. Mounting pressures to replace synthetically derived surfactants with environmentally friendly, low toxicity, bio-surfactants have steadily grown in various applications (e.g. dispersants used in oil spill cleanup). Lipopeptides are naturally occurring compounds that show great promise for sustainable bio-surfactants and are found in a breath of bacterial species including well-studied Bacillus strains. This project aims to chart the diversity of lipopeptides from bacterial genomes and to further develop structure prediction methods to generate targeted leads with desirable properties. Comparative analysis of the genetic diversity of lipopeptides will also aid re-engineering efforts for effective production at industrial scales and reduce dependence on fossil fuels.
Linking the metabolome and genome
The central theme of my research is the integration of metabolome and genome mining tools to accelerate and improve functional annotations of biosynthesis genes and specialized molecules. One the one hand, I am working on improving workflows to maximize the structural information obtained from mass spectrometry fragmentation data. On the other hand, I will develop and extend existing workflows that recognize patterns of co-localized genes in predicted biosynthesis genes clusters. Both the metabolomics and genomics workflows focus on the recognition of molecular substructures as building blocks of more complex natural products. As these tools will provide complementary structural data on the specialized molecules produced by microbes, fungi, and plants, I will finally integrate those workflows to boost natural product discovery.
Pangenomic applications for plants and pathogens
As a result of the advances in NGS sequencing there is a gradual shift from representing a species by a single reference genome sequence to representing it by a pangenome. A pangenome is a data structure containing all genomic variation in a species or population. In my project, we aim to develop pangenomic applications for plants and pathogens to demonstrate the advantages of pangenomes: pangenome-based discoveries and improved efficiency and/or accuracy. We will build upon the existing pangenome framework of PanTools, and exploit its existing features to develop new computational methods for highly efficient comparative analysis of large numbers of related genomes. One line of research focusses on gene-level analyses in large sets of genomes. For example, inferring the evolutionary relationships across thousand bacterial strains or identifying a subset of genes in tomato which contribute to a particular trait like drought tolerance. The other line of research is aimed at discovery and exploration of genome-wide variation, from single-nucleotide polymorphisms to large structural variation.
Linking metagenomics and metatranscriptomics to study the endophytic root microbiome
Like most higher organisms, plants have a microbiome that protects them against diseases and stimulates their growth. In this project, we will study the microbial community that lives inside plant roots. By linking metagenomics and metatranscriptomics, we attempt to find out what the function of these microbes is and how they interact with each other, with pathogens and with the plant. Microbes and plants produce a wide array of secondary metabolites, which are used to interact with other organisms in their environment. Secondary metabolites can function as antibiotics, pigments, and effectors. Additionally, they can help with nutrient acquisition and communication. The genes encoding the pathways for secondary metabolites are often co-localised in clusters on the genome. Over 700 of these so-called biosynthetic gene clusters (BGCs) have been predicted to be present in the endophytic root microbiome. We want to know which BGCs are involved in pathogen suppression and what their exact function is.
Exploiting variation in lettuce and its wild relatives
Advancing in the genome era, sequencing one’s plants for the identification of variation underlying traits of interest is more accessible than ever. Comparing results between species has therefore become important as well (comparative genomics). In this research, which is part of the LettuceKnow project (https://lettuceknow.nl/ ), we use a pangenomic approach to search for genomic and transcriptomic variation across species within the genus Lactuca that can explain traits lettuce breeders are interested in. A pangenome is a hypothetical construct in which multiple fully sequenced genomes are combined into one representative reference genome. Specifically, we aim to develop new functionalities within the pangenomic framework PanTools (which is being developed at Wageningen University) to integrate genomic, transcriptomic and phenotypic data for accurate interspecies comparisons. These functionalities are immediately used for the analysis of the large amounts of sequencing and phenotype data within LettuceKnow, which will be a starting point for collaboration with molecular biologists.