Serum-free mycoplasma growth medium

Gaspari, Erika; Garcia Morales, Luis; Martins Dos Santos, Vitor Alexandre Pires


The present invention provides a serum-free cell culture medium formulation that supports the in vitro cultivation of Mycoplasma species, especially of M. pneumoniae, and methods for cultivating Mycoplasma species in vitro, using these media. The media comprise a basal medium comprising at least one carbon source, vitamins, nucleotides, amino acids, and lipids including cholesterol, phospholipids and sphingolipids, and optionally palmitic acid and/or oleic acid. The invention further provides a use of phospholipids and sphingolipids to support growth of a Mycoplasma spp., preferably M. pneumoniae.