Publications

Development and evaluation of two TaqMan assays for generic detection of Dickeya species

Zijlstra, Carolien; Haas, Lia Groenenboom De; Krijger, Marjon; Verstappen, Els; Warris, Sven; Haan, Eisse de; Wolf, Jan van der

Summary

Two TaqMan assays were developed for generic detection of Dickeya species in plant material. The assays enabled detection of all strains of D. chrysanthemi, D. dadantii, D. dianthicola, D. solani and D. zeae tested, but only weak reactions were found with strains of D. paradisiaca. The assays enabled detection of as low as 100 fg of target DNA in a background of potato DNA. The assays were evaluated with DNA extracted from potato tuber extracts, in which different Dickeya species can be present. Results of both assays were highly similar indicating that they can be used reliably in potato seed testing programs.