Mushroom forming fungi are versatile organisms that are suitable for a wide range of applications. However, compared to plants, there is little research being done and the available knowledge is fragmented. By further improving breeding, we can exploit the true potential of mushroom forming fungi. We are consolidating available knowledge and bring scientists and businesses together. Together we are working on new, economically viable applications for mushroom forming fungi.
Wageningen University & Research has long been a world leader in mushroom breeding. We have a collection of thousands of mushroom-forming fungi. From various strains of these fungi we select the most suitable genetic material for each intended application and then use it in our laboratories for improvement by breeding.
Food and non-food applications
Thanks in part to our research, you can now find a wider variety of edible mushrooms in the refrigerated section of the supermarket. Mushrooms are also being used as ingredients in many food products. Furthermore, new applications are opening up more and more possibilities for using mushroomforming fungi for non-food applications:
- Natural, environmentally friendly technologies for purifying wastewater with fungi are rapidly developing as fully-fledged alternatives to chemical wastewater treatment;
- Enzymes that are produced by certain fungi have been found to be highly effective in breaking down lignin in woody waste streams and releasing valuable sugars;
- Fibres in mushroomforming fungi have a lot of potential as a raw material for products such as sustainable clothing;
- Thanks to their relatively simple genetic structure and shorter life cycles, mushrooms are suitable model organisms for studying complex genetic processes that also occur in plants.
Contributing to high-value commercialisation of mushrooms
With its independent research, Wageningen University & Research promotes high-value commercialisation of mushroom-like fungi. Our fundamental research contributes to a better understanding of their genetics. This knowledge helps us to work with commercial enterprises to develop tailor-made technological routes for new applications.
Our Plant Breeding department has a large collection of fungi that produce edible mushrooms (the collection contains more than 5,000 strains representing more than 125 species), with emphasis on the button mushroom (Agaricus bisporus), the oyster mushroom (Pleurotus spp.), and the shiitake (Lentinula edodes). Currently, additional species of (edible) mushroom forming fungi are actively collected and added.
Unifarm has an experimental, climate-controlled cultivation facility in which edible mushrooms can be produced on pilot scale. The design of the facility produces a crop that is comparable to commercial production and reliable phenotyping (e.g. disease resistance, quality, harvest, etc.). The rooms within the facility are also suitable for closed cultivation of genetically modified strains, and for cultivation of fungi for novel applications.
Substrate (compost) is one of the main cost items in button mushroom production. Research is focused on the role played by vegetative mycelium in the decomposition of organic matter and the formation of mushrooms. This knowledge is used to improve the efficiency of the cultivation system. In addition, the biological variation in the collection is used to improve substrate utilisation by commercial varieties.
The mushroom researchers generate segregating populations for traits that are relevant to primary producers (yield, quality, disease resistance) or consumers (taste, bioactive compounds, etc.). These traits are mapped using SNP markers or GBS (Genotyping By Sequencing). Markers linked to traits are then used for selection in offspring. The sporeless oyster mushroom is one of the products which this research group has cultivated.
The button mushroom is represented by two compatible subspecies differing in the interchromosomal crossovers. In commercial lines and most wild isolates, the crossovers are mainly confined to chromosome ends, whereas in the other subspecies crossovers take place over the entire chromosome. Segregating populations are currently being used to study the mechanisms behind these two recombination landscapes. It is hoped that this will generate knowledge that will enable meiosis to be controlled in such a way that all combinations can either be multiplied or maintained.
Button mushrooms we buy in the shops are almost identical the world over. To find out why, read the article written by Wageningen mushroom expert Anton Sonnenberg for Atlas of Science: Why are all button mushroom cultivars similar?
The utilisation of lignocellulose is limited by the presence of recalcitrant lignin. Physical and chemical pretreatments are now being used to reduce/modify lignin and improve access to (hemi)cellulose. White rot fungi (including many edible fungi) selectively degrade lignin during vegetative growth and can therefore be used to commercialise lignocellulose in a low-tech, low-cost and sustainable way.
Genes that are differentially expressed in vegetative growth and initiation of mushrooms (pins) are overexpressed or knocked out using Atum transformation (via crossover and CRISPR-Cas9). The aim is to unravel the mechanism behind mushroom formation, allowing growers more control over the formation of mushrooms, which in turn could lead to the commercial production of mushroom species that were non-cultivable up to now.
Author Correction: Telomere-to-telomere assembled and centromere annotated genomes of the two main subspecies of the button mushroom Agaricus bisporus reveal especially polymorphic chromosome endsScientific Reports 11 (2021)1. - ISSN 2045-2322
Mating-type locus organization and mating-type chromosome differentiation in the bipolar edible button mushroom agaricus bisporusGenes 12 (2021)7. - ISSN 2073-4425
Data underlying the publication: Mapping recombination landscape and basidial spore number in the button mushroom Agaricus bisporus: Wageningen University & Research
Assessing the nutritional quality of fungal treated wheat straw : Compounds formed after treatment with Ceriporiopsis subvermispora and Lentinula edodesAnimal Feed Science and Technology 276 (2021). - ISSN 0377-8401
Coprophilous fungi : Closing the loop: improving circularity with manure-loving mushroomsWageningen : Stichting Wageningen Research, Wageningen Plant Research, Business unit Plant Breeding (Report / Stichting Wageningen Research, Wageningen Plant Research, Business Unit Plant Breeding WPR-2021-1) - p.
For previous publications of research on mushrooms, fungi and breeding, visit the Mushroom Research unit’s Research@WUR overview page.