Onion genome finally reveals its secrets

The onion is one of the most widely grown vegetables in the world and is full of vitamins and minerals. It is an important component of people’s diets in many countries. “If onions are to be produced all over the world, we need varieties that are suitable for the various growing conditions,” says Olga Scholten, a plant breeding researcher. “For example, we need onions that are resistant to various fungi, as fungal diseases can damage onions and cause considerable crop losses.” Breeding new varieties will be easier now that the onion genome is available.

A puzzle of 100,000 pieces

The size of the onion genome meant that unravelling it was a significant task. “Assembling the onion genome is comparable to completing a puzzle with 100,000 pieces, of which 95,000 are just bits of blue sky,” says Finkers. “Only 5000 pieces really make the difference. We managed to sequence a large proportion with the help of the latest DNA sequencing technologies because with that technology we managed to assemble the small and large pieces that partially overlap.”

“Now that we are publishing the onion genome, other researchers and plant breeders will be able to take advantage of the opportunities presented by the genome sequence,” says Finkers. “This will expand the knowledge base and allow the screening of many more varieties or wild species for the presence of particular genes or to combine them. This could be included in a cross-breeding programme in which useful characteristics from other onion varieties or from related wild species will be combined.”

Plant breeders expect that access to the onion genome sequence will double the speed of their breeding work, in the future bringing it down to about six or seven years. “In other crops, such as potatoes and rice, the role that certain genes play in providing resistance to disease or tolerance to drought has already been thoroughly studied. Based on the sequence information from genes in those crops, plant breeders can now more easily identify similar genes in the onion genome and more accurately make progress in their own breeding work.”

Collaboration

Wageningen University & Research collaborated with three companies on sequencing the onion's DNA: the plant breeding companies Bejo Zaden and De Groot en Slot, and the genomics company ServiceXS. Parts of the project were completed through academic collaboration with Prof. Masayoshi Shigyo (Laboratory of Vegetable Crop Science, Yamaguchi University, Japan) and Prof. Shusei Sato (Graduate School of Life Sciences, Tohoku University, Aoba-ku, Sendai, Japan).